Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle
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چکیده
منابع مشابه
Vacuolar H+-ATPase in ocular ciliary epithelium.
The mechanisms controlling the production of aqueous humor and the regulation of intraocular pressure are poorly understood. Here, we provide evidence that a vacuolar H+-ATPase (V-ATPase) in the ocular ciliary epithelium is a key component of this process. In intracellular pH (pHi) measurements of isolated ciliary epithelium performed with 2',7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF), t...
متن کاملAMP-activated protein kinase inhibits alkaline pH- and PKA-induced apical vacuolar H+-ATPase accumulation in epididymal clear cells.
Acidic luminal pH and low [HCO(3)(-)] maintain sperm quiescent during maturation in the epididymis. The vacuolar H(+)-ATPase (V-ATPase) in clear cells is a major contributor to epididymal luminal acidification. We have shown previously that protein kinase A (PKA), acting downstream of soluble adenylyl cyclase stimulation by alkaline luminal pH or HCO(3)(-), induces V-ATPase apical membrane accu...
متن کاملThe Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals
In this study, the effect of metabolic inhibition (MI) by glucose substitution with 2-deoxyglucose (2-DOG) and/or application of antimycin A on ovine rumen epithelial cells (REC) vacuolar-type H(+)-ATPase (vH(+)-ATPase) activity was investigated. Using fluorescent spectroscopy, basal pH(i) of REC was measured to be 7.3 +/- 0.1 in HCO(3) (-)-free, glucose-containing NaCl medium. MI induced a str...
متن کاملVacuolar H+-ATPase, but not mitochondrial F1F0-ATPase, is required for aluminum resistance in Saccharomyces cerevisiae.
It was recently shown that vacuolar ATPase and mitochondrial F1F0-ATPase activities are induced by aluminum (Al) in an Al-resistant cultivar of wheat, suggesting that induction of these enzymes could be an adaptive trait involved in Al resistance. To test this hypothesis, we used the Saccharomyces cerevisiae model system. In yeast, unlike wheat, the activity, transcript and protein levels of mi...
متن کاملRenal vacuolar H+-ATPase.
Vacuolar H(+)-ATPases are ubiquitous multisubunit complexes mediating the ATP-dependent transport of protons. In addition to their role in acidifying the lumen of various intracellular organelles, vacuolar H(+)-ATPases fulfill special tasks in the kidney. Vacuolar H(+)-ATPases are expressed in the plasma membrane in the kidney almost along the entire length of the nephron with apical and/or bas...
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ژورنال
عنوان ژورنال: Journal of Animal Science and Technology
سال: 2021
ISSN: 2672-0191,2055-0391
DOI: 10.5187/jast.2021.e32